GeneTex

  • Country / Location Selection

United States (US)

DCUN1D1 antibody [N2C3]

Anti-DCUN1D1 antibody [N2C3] used in Western Blot (WB). GTX116558

Cat. No. GTX116558

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DCUN1D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCUN1D1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616556

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

defective in cullin neddylation 1 domain containing 1 , DCNL1 , DCUN1L1 , RP42 , SCCRO , SCRO , Tes3

Background

Part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression.

Database

Research Area

DATA IMAGES

Anti-DCUN1D1 antibody [N2C3] used in Western Blot (WB). GTX116558

GTX116558 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
C: Raji
12% SDS PAGE
GTX116558 diluted at 1:1000

REFERENCE

Application Reference

Wang J et al. Clin Cancer Res 2013; 19 (20) : 5580-90 Integrative genomics analysis identifies candidate drivers at 3q26-29 amplicon in squamous cell carcinoma of the lung.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for DCUN1D1 antibody [N2C3] (GTX116558). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169