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DDAH1 antibody [C2C3], C-term

Anti-DDAH1 antibody [C2C3], C-term used in Western Blot (WB). GTX104660
Anti-DDAH1 antibody [C2C3], C-term used in Western Blot (WB). GTX104660
Anti-DDAH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104660

Cat. No. GTX104660

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DDAH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Raji , mouse kidney

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDAH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240691

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dimethylarginine dimethylaminohydrolase 1 , DDAH , DDAH-1 , DDAHI , HEL-S-16

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDAH1 antibody [C2C3], C-term used in Western Blot (WB). GTX104660

GTX104660 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DDAH1 antibody [C2C3], C-term (GTX104660) diluted at 1:1000.

Anti-DDAH1 antibody [C2C3], C-term used in Western Blot (WB). GTX104660

GTX104660 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX104660 diluted at 1:1000

Anti-DDAH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104660

GTX104660 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using DDAH1(GTX104660) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169