GeneTex
United States (US)

DDAH1 antibody [C2C3], C-term

GTX104660_WB_19011506_355.jpg
GTX104660_39708_IFA_19011506_256.jpg
GTX104660_39708_20161229_WB_M_kidney_19011506_676.jpg

Cat No. GTX104660

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Raji , mouse kidney

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDAH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDAH antibody, FLJ21264 antibody, FLJ25539 antibody, DDAH1 antibody, DDAH-1 antibody, "NG, NG-dimethylarginine dimethylaminohydrolase antibody", dimethylargininase-1 antibody, "N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 antibody", DDAHI antibody, dimethylarginine dimethylaminohydrolase 1 antibody

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq]

Database

DATA IMAGES
GTX104660_WB_19011506_355.jpg

GTX104660 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX104660 diluted at 1:1000

GTX104660_39708_IFA_19011506_256.jpg

GTX104660 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using DDAH1(GTX104660) antibody at 1:500 dilution.

GTX104660_39708_20161229_WB_M_kidney_19011506_676.jpg

GTX104660 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DDAH1 antibody [C2C3], C-term (GTX104660) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169