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DDAH2 antibody [N2C3]

Anti-DDAH2 antibody [N2C3] used in Western Blot (WB). GTX116339
Anti-DDAH2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116339
Anti-DDAH2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116339

Cat. No. GTX116339

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DDAH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDAH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618385

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dimethylarginine dimethylaminohydrolase 2 , DDAH , DDAHII , G6a , HEL-S-277 , NG30

Background

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDAH2 antibody [N2C3] used in Western Blot (WB). GTX116339

GTX116339 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX116339 diluted at 1:1000

Anti-DDAH2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116339

GTX116339 ICC/IF Image

DDAH2 antibody [N2C3] detects DDAH2 protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 15 min.
Green: DDAH2 protein stained by DDAH2 antibody [N2C3] (GTX116339) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DDAH2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116339

GTX116339 IHC-P Image

DDAH2 antibody [N2C3] detects DDAH2 protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
DDAH2 antibody [N2C3] (GTX116339) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169