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DDB1 antibody [1322]

Anti-DDB1 antibody [1322] used in Western Blot (WB). GTX70302
Anti-DDB1 antibody [1322] used in Western Blot (WB). GTX70302

Cat. No. GTX70302

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1322

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all DDB1 products

APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For WB(ECL): Use at a dilution of 1:1,000 in 5% Milk/PBS containing 0.05% Tween. We recommend an overnight incubation at 4¡C. Secondary antibody (GTX30590) 2 hours at RT with dilution of 1:5,000 in 5% Milk/PBS containing 0.05% Tween. Not tested in other applictions. Optiamal dilutions/concentrations should be determined by the researcher.

Calculated MW

127 kDa. ( Note )

Positive Control

T47D and MCF-7 whole cell lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide congugated to protein carrier. The mice were immunized with a combination of N & C peptide. The Swiss Prot ID is Q16531.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369931

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-DDB1 antibody [1322] used in Western Blot (WB). GTX70302

GTX70302 WB Image

Anti-DDB1 antibody [1322] used in Western Blot (WB). GTX70302

GTX70302 WB Image

Western blot using GTX70302 - Detection of Human DDB1 protein by western blot experiment in T47D whole cell lysate by GTX70302.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
Custom Conjugations