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DDB1 antibody [C3], C-term

KO/KD
Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232
Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232
Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232
Anti-DDB1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100232
Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232
Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232
Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232
Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232
Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232

Cat No. GTX100232

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all DDB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

293T , A549 , H1299 , HCT116 , MCF-7 , Neuro2A , GL261 , C8D30 , PC-12

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036745

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232

GTX100232 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [C3], C-term (GTX100232) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232

GTX100232 WB Image

Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232

GTX100232 IHC-P Image

DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDB1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100232

GTX100232 ICC/IF Image

DDB1 antibody [C3], C-term detects DDB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DDB1 protein stained by DDB1 antibody [C3], C-term (GTX100232) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232

GTX100232 WB Image

Sample (30 μg of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232

GTX100232 IHC-P Image

DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232

GTX100232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using DDB1(GTX100232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDB1 antibody [C3], C-term used in Western Blot (WB). GTX100232

GTX100232 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDB1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100232

GTX100232 IHC-P Image

DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yasukawa T et al. Cell Rep 2020; 30 (10) : 3478-3491.e6 NRBP1-Containing CRL2/CRL4A Regulates Amyloid β Production by Targeting BRI2 and BRI3 for Degradation.
Application : WB
Reactivity : Human
Moser B et al. Cells 2020; 9 (2) Comparative Interactome Analysis of Emerin, MAN1 and LEM2 Reveals a Unique Role for LEM2 in Nucleotide Excision Repair.
Application : WB
Reactivity : Human
Guo H et al. Biochem Biophys Res Commun 2019; 513 (4) : 933-939 Determinants of lentiviral Vpx-CRL4 E3 ligase-mediated SAMHD1 degradation in the substrate adaptor protein DCAF1.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169