APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , HCT116 , MCF-7 , Neuro2A , GL261 , C8D30 , PC-12 , 293T
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2036745
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF
Cellular Localization
Cytoplasm , Nucleus
Background
This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100232 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [C3], C-term (GTX100232) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100232 WB Image
Sample (30 μg of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE GTX100232 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100232 WB Image
Sample (30 μg of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7.5% SDS PAGE GTX100232 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100232 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using DDB1(GTX100232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100232 IHC-P Image
DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100232 WB Image
Sample (30 μg of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX100232 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100232 ICC/IF Image
DDB1 antibody [C3], C-term detects DDB1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DDB1 protein stained by DDB1 antibody [C3], C-term (GTX100232) diluted at 1:500. Blue: Hoechst 33343 staining.
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GTX100232 IHC-P Image
DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100232 IHC-P Image
DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100232 WB Image
The data was published in the journal Cells in 2020.PMID: 32085595
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REFERENCE
REVIEW
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