Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [C3], C-term (GTX100232) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

DDB1 antibody [C3], C-term detects DDB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DDB1 protein stained by DDB1 antibody [C3], C-term (GTX100232) diluted at 1:500.
Blue: Hoechst 33343 staining.

Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using DDB1(GTX100232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Cells in 2020.PMID: 32085595