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DDB1 antibody [N1N3]

KO/KD
Anti-DDB1 antibody [N1N3] used in Western Blot (WB). GTX100129
Anti-DDB1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100129
Anti-DDB1 antibody [N1N3] used in Western Blot (WB). GTX100129
Anti-DDB1 antibody [N1N3] used in Western Blot (WB). GTX100129

Cat. No. GTX100129

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DDB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

Mouse brain , HeLaS3 , Raji , 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240693

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDB1 antibody [N1N3] used in Western Blot (WB). GTX100129

GTX100129 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [N1N3] (GTX100129) diluted at 1:5000.

Anti-DDB1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100129

GTX100129 ICC/IF Image

DDB1 antibody [N1N3] detects DDB1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDB1 protein stained by DDB1 antibody [N1N3] (GTX100129) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DDB1 antibody [N1N3] used in Western Blot (WB). GTX100129

GTX100129 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX100129 diluted at 1:1000

Anti-DDB1 antibody [N1N3] used in Western Blot (WB). GTX100129

GTX100129 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
B:Raji(GTX27908)
7.5% SDS PAGE
GTX100129 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169