APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , HeLaS3 , Raji , 293T
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DDB1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240693
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF
Cellular Localization
Cytoplasm , Nucleus
Background
This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100129 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [N1N3] (GTX100129) diluted at 1:5000.
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GTX100129 ICC/IF Image
DDB1 antibody [N1N3] detects DDB1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDB1 protein stained by DDB1 antibody [N1N3] (GTX100129) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX100129 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX100129 diluted at 1:1000
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GTX100129 WB Image
Sample(30 μg of whole cell lysate) A:HeLa S3 (GTX14654) B:Raji(GTX27908) 7.5% SDS PAGE GTX100129 diluted at 1:1000
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REFERENCE
There are currently no references for DDB1 antibody [N1N3] (GTX100129). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DDB1 antibody [N1N3] (GTX100129). Be the first to share your experience with this product.