GeneTex
United States (US)

DDB1 antibody [N1N3]

KO/KD
GTX100129_39384_20160728_WB_shRNA_watermark_19011506_362.jpg
GTX100129_WB_19011506_675.jpg
GTX100129_39384_WB_M_Brain_19011506_255.jpg
GTX100129_39384_IFA_19011506_128.jpg

Cat No. GTX100129

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

293T , HeLaS3 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, DNA damage-binding protein a antibody, XPE-binding factor antibody, UV-DDB 1 antibody, xeroderma pigmentosum group E-complementing protein antibody, UV-damaged DNA-binding protein 1 antibody, DNA damage-binding protein 1 antibody, XAP-1 antibody, HBV X-associated protein 1 antibody, DDB p127 subunit antibody, UV-damaged DNA-binding factor antibody, damage-specific DNA-binding protein 1 antibody, "damage-specific DNA binding protein 1, 127kDa antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100129_39384_20160728_WB_shRNA_watermark_19011506_362.jpg

GTX100129 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [N1N3] (GTX100129) diluted at 1:5000.

GTX100129_WB_19011506_675.jpg

GTX100129 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
B:Raji(GTX27908)
7.5% SDS PAGE
GTX100129 diluted at 1:1000

GTX100129_39384_WB_M_Brain_19011506_255.jpg

GTX100129 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX100129 diluted at 1:1000

GTX100129_39384_IFA_19011506_128.jpg

GTX100129 ICC/IF Image

DDB1 antibody [N1N3] detects DDB1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDB1 protein stained by DDB1 antibody [N1N3] (GTX100129) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169