GeneTex
United States (US)

DDB1 antibody

KO/KD
GTX100130_40073_IP_19011506_619.jpg
GTX100130_IHC_19011506_474.jpg
GTX100130_40073_20160728_WB_shRNA_watermark_19011506_886.jpg
GTX100130_40073_WB_M_brain_19011506_372.jpg
GTX100130_40073_IHC_R_19011506_800.jpg
GTX100130_40073_20160818_WB_R_19011506_650.jpg
GTX100130_WB_M_19011506_166.jpg
GTX100130_WB_19011506_816.jpg
GTX100130_IFA_19011506_848.jpg
GTX100130_40073_20160818_WB_R_brain_19011506_438.jpg
GTX100130_40073_IHC_M_19011506_892.jpg

Cat No. GTX100130

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

293T , HepG2 , NIH-3T3 , mouse brain , PC-12 , Rat2 , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Xenopus tropicalis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, DNA damage-binding protein a antibody, XPE-binding factor antibody, UV-DDB 1 antibody, xeroderma pigmentosum group E-complementing protein antibody, UV-damaged DNA-binding protein 1 antibody, DNA damage-binding protein 1 antibody, XAP-1 antibody, HBV X-associated protein 1 antibody, DDB p127 subunit antibody, UV-damaged DNA-binding factor antibody, damage-specific DNA-binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100130_40073_IP_19011506_619.jpg

GTX100130 IP Image

DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 μg of DDB1 antibody (GTX100130) 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody (GTX100130) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100130_IHC_19011506_474.jpg

GTX100130 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1(GTX100130) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100130_40073_20160728_WB_shRNA_watermark_19011506_886.jpg

GTX100130 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130_40073_WB_M_brain_19011506_372.jpg

GTX100130 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX100130 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130_40073_IHC_R_19011506_800.jpg

GTX100130 IHC-P Image

DDB1 antibody detects DDB1 protein at nucleus and cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
DDB1 antibody (GTX100130) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100130_40073_20160818_WB_R_19011506_650.jpg

GTX100130 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130_WB_M_19011506_166.jpg

GTX100130 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100130 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130_WB_19011506_816.jpg

GTX100130 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX100130 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130_IFA_19011506_848.jpg

GTX100130 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1(GTX100130) antibody at 1:200 dilution.

GTX100130_40073_20160818_WB_R_brain_19011506_438.jpg

GTX100130 WB Image

Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX100130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100130_40073_IHC_M_19011506_892.jpg

GTX100130 IHC-P Image

DDB1 antibody detects DDB1 protein at nucleus and cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
DDB1 antibody (GTX100130) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169