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DDB1 antibody

Anti-DDB1 antibody used in Western Blot (WB). GTX70307
Anti-DDB1 antibody used in Western Blot (WB). GTX70307

Cat No. GTX70307

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

For WB: Use at an assay dependent dilution. Predicted molecular weight: 127 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

127 kDa. ( Note )

Positive Control

T47D and MCF-7 whole cell lysate

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide conugated to protein carrier.

Purification

Affinity purified with antigen
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Damage Specific Dna Binding Protein 1 , Ddba , Uv-Ddb1 , Xap1 , Xpce , Xpe , Xpe-Bf , Ddb1

Background

The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

Database

Research Area

DATA IMAGES

Anti-DDB1 antibody used in Western Blot (WB). GTX70307

GTX70307 WB Image

Anti-DDB1 antibody used in Western Blot (WB). GTX70307

GTX70307 WB Image

Detection of Human DDB1 in (1) Jurkat, (2) MCF7, and (3) T47D cell lysates using GeneTex polyclonal antibody GTX70307

REFERENCE

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Package List Price ($)
$ 329