Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX70307) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Detection of Human DDB1 in (1) Jurkat, (2) MCF7, and (3) T47D cell lysates using GeneTex polyclonal antibody GTX70307