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DDDDK tag antibody [F.G2]

Anti-DDDDK tag antibody [F.G2] used in Western Blot (WB). GTX80656

Cat No. GTX80656

Host

Mouse

Clonality

Monoclonal

Clone Name

F.G2

Isotype

IgG2b

Application

WB, ICC/IF, IP
Package
100 μg ($229)

APPLICATION

Application Note

For IF: Use at a dilution of 1:200. For IP: Use at a dilution of 1:200. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A synthetic peptide (DYKDDDDK) coupled to KLH.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Research Area

DATA IMAGES

Anti-DDDDK tag antibody [F.G2] used in Western Blot (WB). GTX80656

GTX80656 WB Image

Western Blot analysis of 293 cells transfected with DYKDDDK-tagged vectors at different concentration(0.5mg/ml),
Lane 4:lowest concentration(0.1ug/ml)

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 229