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DDDDK tag antibody [F.G2]

Anti-DDDDK tag antibody [F.G2] used in Western Blot (WB). GTX80656

Cat. No. GTX80656

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F.G2

Isotype

 IgG2b

Application

 WB, ICC/IF, IP

Reactivity

 Species independent
Package
100 μg ($399)
See all DDDDK tag products

APPLICATION

Application Note

For IF: Use at a dilution of 1:200. For IP: Use at a dilution of 1:200. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Product Note

Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A synthetic peptide (DYKDDDDK) coupled to KLH.

Conjugation

Unconjugated

RRID

AB_625874

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Research Area

DATA IMAGES

Anti-DDDDK tag antibody [F.G2] used in Western Blot (WB). GTX80656

GTX80656 WB Image

Western Blot analysis of 293 cells transfected with DYKDDDK-tagged vectors at different concentration(0.5mg/ml),
Lane 4:lowest concentration(0.1ug/ml)

REFERENCE

Application Reference

Li Z et al. Sci Rep 2018; 8 (1) : 11038 Exposure to galactic cosmic radiation compromises DNA repair and increases the potential for oncogenic chromosomal rearrangement in bronchial epithelial cells.
Chen Z et al. Nucleic Acids Res 2013; 41 (19) : e182 Receptor-mediated delivery of engineered nucleases for genome modification.
Application : ICC/IF
Sud N et al. Am J Physiol Lung Cell Mol Physiol 2008; 294 (3) : L582-91 Protein kinase Cdelta regulates endothelial nitric oxide synthase expression via Akt activation and nitric oxide generation.
Application : WB
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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 399