For IF: Use at a dilution of 1:200. For IP: Use at a dilution of 1:200. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.
Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Phosphate-buffered saline, pH 7.2, containing 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
A synthetic peptide (DYKDDDDK) coupled to KLH.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.