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DDDDK tag antibody [FG4R]

Anti-DDDDK tag antibody [FG4R] used in Western Blot (WB). GTX82562

Cat No. GTX82562

Host

Mouse

Clonality

Monoclonal

Clone Name

FG4R

Isotype

IgG1

Application

WB, ICC/IF, IP, ELISA, IHC
Package
100 μg ($379)

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

DYKDDDDK (FLAG) synthetic peptide conjugated to KLH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11165512

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FLAG Tag , DDDDK Tag

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Research Area

DATA IMAGES

Anti-DDDDK tag antibody [FG4R] used in Western Blot (WB). GTX82562

GTX82562 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)

REFERENCE

REVIEW

DDDDK tag antibody [FG4R] Cat No. GTX82562

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Jul-2021
Score :
Application Tested : WB
Sample : HEK293T cells
Amount used : 10
Blocking : 5% skim milk/0.05% Tween20 in TBS, 25°C, 2Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: vector (3xFLAG CMV)
Lane 2: mouse PLD3-3xFLAG
Lane 3: mouse PLD3 luminal-3xFLAG
SDS
PBS.pdf
Package List Price ($)
$ 379
Custom Conjugations