DDDDK tag antibody [FG4R]

Name: DDDDK tag antibody [FG4R]
Brand: GeneTex
SKU: GTX82562
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-DDDDK tag antibody [FG4R] used in Western Blot (WB). GTX82562

Cat. No. GTX82562

Reference 2

Host	Mouse
Clonality	Monoclonal
Clone Name	FG4R
Isotype	IgG1
Application	WB, ICC/IF, IP, ELISA, IHC
Reactivity	Species independent
Package	100 μg ($399)

See all DDDDK tag products

View product citations for antibody GTX82562 on CiteAb

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

DYKDDDDK (FLAG) synthetic peptide conjugated to KLH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11165512

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FLAG Tag , DDDDK Tag

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Research Area

DATA IMAGES

GTX82562 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)

REFERENCE

Application Reference

Schulte JE et al. mBio 2018; 9 (6) The Phosphohistidine Phosphatase SixA Targets a Phosphotransferase System.

Wu HC et al. Biotechnol Prog 2015; Production and characterization of active recombinant interleukin-12/eGFP fusion protein in stably-transfected DF1 chicken cells.

Application : ELISA

Submit a Reference

REVIEW