DDDDK tag antibody [GT1423] immunoprecipitates DDDK-tagged protein in IP experiments.
IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells.
A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells
B : Control with 2.5 μg of pre-immune mouse IgG
C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT1423] (GTX629632)
7.5% SDS-PAGE
The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT1423] (GTX629632) diluted at 1 : 5000.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

DDDDK tag antibody [GT1423] (GTX629632) detects DDDDK-tagged proteins by western blot.
A. Untransfected 293T whole cell lysate/extract (30 μg)
B.-D. Whole cell lysate/extracts (30 μg) from transfected 293T cells expressing different DDDDK-tagged proteins.
10% SDS-PAGE
DDDDK tag antibody [GT1423] (GTX629632) dilution: 1:5000