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DDDDK tag antibody [GT1423]

Anti-DDDDK tag antibody [GT1423] used in Immunoprecipitation (IP). GTX629632
Anti-DDDDK tag antibody [GT1423] used in Western Blot (WB). GTX629632

Cat. No. GTX629632

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1423

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Positive Control

DDDDK-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888127

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DDDDK tag antibody

Research Area

DATA IMAGES

Anti-DDDDK tag antibody [GT1423] used in Immunoprecipitation (IP). GTX629632

GTX629632 IP Image

DDDDK tag antibody [GT1423] immunoprecipitates DDDK-tagged protein in IP experiments.
IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells.
A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells
B : Control with 2.5 μg of pre-immune mouse IgG
C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT1423] (GTX629632)
7.5% SDS-PAGE
The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT1423] (GTX629632) diluted at 1 : 5000.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
Anti-DDDDK tag antibody [GT1423] used in Western Blot (WB). GTX629632

GTX629632 WB Image

DDDDK tag antibody [GT1423] (GTX629632) detects DDDDK-tagged proteins by western blot.
A. Untransfected 293T whole cell lysate/extract (30 μg)
B.-D. Whole cell lysate/extracts (30 μg) from transfected 293T cells expressing different DDDDK-tagged proteins.
10% SDS-PAGE
DDDDK tag antibody [GT1423] (GTX629632) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations