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DDDDK tag antibody [GT231]

Anti-DDDDK tag antibody [GT231] used in Immunoprecipitation (IP). GTX629631
Anti-DDDDK tag antibody [GT231] used in Western Blot (WB). GTX629631

Cat No. GTX629631

Host

Mouse

Clonality

Monoclonal

Clone Name

GT231

Isotype

IgG2a

Application

WB, IP
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Positive Control

DDDDK-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DDDDK tag antibody

Research Area

DATA IMAGES

Anti-DDDDK tag antibody [GT231] used in Immunoprecipitation (IP). GTX629631

GTX629631 IP Image

DDDDK tag antibody [GT231] immunoprecipitates DDDK-tagged protein in IP experiments.
IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells.
A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells
B : Control with 2.5 μg of pre-immune mouse IgG
C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT231] (GTX629631)
7.5% SDS-PAGE
The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT231] (GTX629631) diluted at 1 : 5000.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-DDDDK tag antibody [GT231] used in Western Blot (WB). GTX629631

GTX629631 WB Image

DDDDK tag antibody [GT231] (GTX629631) detects DDDDK-tagged proteins by western blot.
A. Untransfected 293T whole cell lysate/extract (30 μg )
B.-D. Whole cell lysate/extracts (30 μg) from transfected 293T cells expressing different DDDDK-tagged proteins.
10% SDS-PAGE
DDDDK tag antibody [GT231] (GTX629631) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

DDDDK tag antibody [GT231] Cat No. GTX629631

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Jul-2014
Score :
Application Tested : WB
Sample : DDDDK-tagged protein expressing human mammary epithelial cells
Amount used : 30μg
Blocking : 5% skimmilk in TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, 18Hr
Lane Description : Lane A:Untransfected human mammary epithelial cells, Lane B:DDDDK-tagged protein expressing human mammary epithelial cells
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169