DDDDK tag antibody [GT482]

Cat. No. GTX636631

Host	Mouse
Clonality	Monoclonal
Clone Name	GT482
Isotype	IgG2b
Application	WB, ICC/IF, IP
Package	100 μl ($399), 25 μl ($169)

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Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910016

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX636631 ICC/IF Image

DDDDK tag antibody [GT482] detects DDDDK tag protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody [GT482] (GTX636631) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GTX636631 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT482] (GTX636631) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX636631 IP Image

Immunoprecipitation of DDDDK-tag tagged protein from DDDDK-tag tagged protein transfected 293T whole cell extracts using 5 μg of DDDDK tag antibody [GT482] (GTX636631).
Western blot analysis was performed using DDDDK tag antibody [GT482] (GTX636631).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

DDDDK tag antibody [GT482]

Cat. No. GTX636631

Host

Clonality

Clone Name

Isotype

Application