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DDDDK tag antibody [GT482]

Anti-DDDDK tag antibody [GT482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636631
Anti-DDDDK tag antibody [GT482] used in Immunoprecipitation (IP). GTX636631
Anti-DDDDK tag antibody [GT482] used in Western Blot (WB). GTX636631

Cat No. GTX636631

Host

Mouse

Clonality

Monoclonal

Clone Name

GT482

Isotype

IgG2b

Application

WB, ICC/IF, IP
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910016

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-DDDDK tag antibody [GT482] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636631

GTX636631 ICC/IF Image

DDDDK tag antibody [GT482] detects DDDDK tag protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody [GT482] (GTX636631) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-DDDDK tag antibody [GT482] used in Immunoprecipitation (IP). GTX636631

GTX636631 IP Image

Immunoprecipitation of DDDDK-tag tagged protein from DDDDK-tag tagged protein transfected 293T whole cell extracts using 5 μg of DDDDK tag antibody [GT482] (GTX636631).
Western blot analysis was performed using DDDDK tag antibody [GT482] (GTX636631).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

Anti-DDDDK tag antibody [GT482] used in Western Blot (WB). GTX636631

GTX636631 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT482] (GTX636631) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169