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DDDDK tag antibody (HRP)

Anti-DDDDK tag antibody (HRP) used in ELISA (ELISA). GTX77454

Cat No. GTX77454

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA, IHC
Package
100 μg ($379)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 0.05ug/ml
ELISA 0.1 - 0.01 ug/ml
IHC 1.0 - 5.0 ug/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

50mM HEPES, stabilizer

Preservative

0.002% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with DDDDK peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, eggs were collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support then conjugated to HRP

Purification

Purified by antigen-affinity chromatography

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_379868

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-DDDDK tag antibody (HRP) used in ELISA (ELISA). GTX77454

GTX77454 ELISA Image

ELISA analysis of immunogen using GTX77454 DDDDK tag antibody (HRP).

REFERENCE

Application Reference

Ercu M et al. Circulation 2020; 142 (2) : 133-149 Phosphodiesterase 3A and Arterial Hypertension.
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SDS
Thimerosal.pdf
Package List Price ($)
$ 379