DDDDK tag antibody [M2-MS]

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with DDDDK tag antibody [M2-MS] (GTX641655) diluted at 1:5000 and competitor's antibody (Highly cited antibody) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

DDDDK tag antibody [M2-MS] detects DDDDK tag protein by immunofluorescent analysis.
Sample: Transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: DDDDK tag stained by DDDDK tag antibody [M2-MS] (GTX641655) diluted at 1:500 and competitor's antibody (Highly Cited Competitor) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below.

Capture: DDDDK tag antibody [M2-MS] (GTX641655) (5 μg/mL)
Detection: Human parvovirus B19 Capsid protein VP1 + VP2 antibody [HL5562] (GTX646236) (1 μg/mL)

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [M2-MS] (GTX641655) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.