DDDDK tag antibody [M2-RB]

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with DDDDK tag antibody [M2-RB] (GTX640415) diluted at 1:10000 and competitor's antibody (Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [M2-RB] (GTX640415) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DDDDK tag antibody [M2-RB] detects DDDDK tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: DDDDK tag stained by DDDDK tag antibody [M2-RB] (GTX640415) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control (GST-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130343-pro) (72.73-1.14 nM). Coated protein was probed with DDDDK tag antibody [HL3005] (GTX640415) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [HL3005] (GTX640415) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.