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DDDDK tag antibody

Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043
Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115043
Anti-DDDDK tag antibody used in ELISA (ELISA). GTX115043

Cat No. GTX115043

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

Transfected lysate

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DDDDK tag antibody , DDDDK epitope tag antibody , DYKDDDDK epitope tag antibody , DYKDDDDK tag antibody , ECS epitope tag antibody , ECS tag antibody , Enterokinase Cleavage Site epitope tag antibody , Flag tag antibody

Research Area

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043

GTX115043 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with DDDDK tag antibody (GTX115043) diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115043

GTX115043 ICC/IF Image

DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody (GTX115043) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DDDDK tag antibody used in ELISA (ELISA). GTX115043

GTX115043 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody (GTX115043) at concentration ranged from 1.95 to 125 ng/mL.

REFERENCE

REVIEW

DDDDK tag antibody Cat No. GTX115043

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was pear reviewed and published in the journal Front Microbiol in 2019.PMID: 31969874
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Viruses in 2019.PMID: 31461934
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB,IP
Figure Note : The data was pear reviewed and published in the journal Viruses in 2019.PMID: 31461934
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169