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DDDDK tag antibody

Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043
Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115043
Anti-DDDDK tag antibody used in ELISA (ELISA). GTX115043

Cat No. GTX115043

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, ELISA
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

Transfected lysate

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166662

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DDDDK tag antibody , DDDDK epitope tag antibody , DYKDDDDK epitope tag antibody , DYKDDDDK tag antibody , ECS epitope tag antibody , ECS tag antibody , Enterokinase Cleavage Site epitope tag antibody , Flag tag antibody

Research Area

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043

GTX115043 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with DDDDK tag antibody (GTX115043) diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115043

GTX115043 ICC/IF Image

DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody (GTX115043) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DDDDK tag antibody used in ELISA (ELISA). GTX115043

GTX115043 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody (GTX115043) at concentration ranged from 1.95 to 125 ng/mL.

REFERENCE

REVIEW

DDDDK tag antibody Cat No. GTX115043

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal Viruses in 2019. PMID: 31461934
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal Viruses in 2019. PMID: 31461934
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019. PMID: 31969874
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169