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DDDDK tag antibody

Comparison
Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043
Anti-DDDDK tag antibody used in ELISA (ELISA). GTX115043
Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115043
Anti-DDDDK tag antibody used in Western Blot and Immunoprecipitation (WB IP). GTX115043
Anti-DDDDK tag antibody used in Western Blot and Immunoprecipitation (WB IP). GTX115043
Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043

Cat. No. GTX115043

Reference 22

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, ELISA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166662

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DDDDK tag antibody , DDDDK epitope tag antibody , DYKDDDDK epitope tag antibody , DYKDDDDK tag antibody , ECS epitope tag antibody , ECS tag antibody , Enterokinase Cleavage Site epitope tag antibody , Flag tag antibody

Research Area

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043

GTX115043 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with DDDDK tag antibody (GTX115043) diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-DDDDK tag antibody used in ELISA (ELISA). GTX115043

GTX115043 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody (GTX115043) at concentration ranged from 1.95 to 125 ng/mL.

Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115043

GTX115043 ICC/IF Image

DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody (GTX115043) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DDDDK tag antibody used in Western Blot and Immunoprecipitation (WB IP). GTX115043

GTX115043 WB IP Image

The data was published in the journal Viruses in 2019. PMID: 31461934

Anti-DDDDK tag antibody used in Western Blot and Immunoprecipitation (WB IP). GTX115043

GTX115043 WB IP Image

The data was published in the journal Viruses in 2019. PMID: 31461934

Anti-DDDDK tag antibody used in Western Blot (WB). GTX115043

GTX115043 WB Image

The data was published in the journal Front Microbiol in 2019. PMID: 31969874

REFERENCE

Application Reference

Zhang MQ et al. Autophagy 2024; 20 (10) : 2238-2254 ATG10S promotes IFNL1 expression and autophagic degradation of multiple viral proteins mediated by IFNL1.
SC HUNG et al. Research Square 2024; TRIM37 maintains 5hmC levels and stemness through preventing DNMT1 alternative splicing
Application : ICC/IF
Dong Y et al. Sci Adv 2024; 10 (8) : eadk4694 Single-cell chromatin profiling reveals genetic programs activating proregenerative states in nonmyocyte cells.
Lai JH et al. Sci Rep 2023; 13 (1) : 20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Zhao B et al. Cell Oncol (Dordr) 2023; 46 (3) : 717-733 USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination.
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REVIEW

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Application Reference
Zhang MQ et al. Autophagy 2024; 20 (10):2238-2254 ATG10S promotes IFNL1 expression and autophagic degradation of multiple viral proteins mediated by IFNL1.
SC HUNG et al. Research Square 2024; TRIM37 maintains 5hmC levels and stemness through preventing DNMT1 alternative splicing
Application : ICC/IF
Dong Y et al. Sci Adv 2024; 10 (8):eadk4694 Single-cell chromatin profiling reveals genetic programs activating proregenerative states in nonmyocyte cells.
Lai JH et al. Sci Rep 2023; 13 (1):20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Zhao B et al. Cell Oncol (Dordr) 2023; 46 (3):717-733 USP13 promotes breast cancer metastasis through FBXL14-induced Twist1 ubiquitination.
SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23):00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B
Application : WB
Himadri Biswas et al. Front Cell Dev Biol 2022; 10:826576 Prolyl Isomerization-Mediated Conformational Changes Define ATR Subcellular Compartment-Specific Functions.
Application : WB, IP
Lu H.A. et al. Circ Res. 2022; 130 (1):112-129 Alternative Splicing Mediated by RNA-Binding Protein RBM24 Facilitates Cardiac Myofibrillogenesis in a Differentiation Stage-Specific Manner.
Application : WB
Wang SA et al. Cell Death Differ 2021; (Epub) USP24 promotes drug resistance during cancer therapy.
Application : WB
Chung-Chi Liao et al. EMBO Rep 2020; 21 (7):e48324 Karyopherin Kap114p-mediated trans-repression controls ribosomal gene expression under saline stress.
Application : WB
Chen X et al. Hum Gene Ther 2020; 31 (7-8):440-447 Different Serotypes of Adeno-Associated Virus Vector- and Lentivirus-Mediated Tropism in Choroid Plexus by Intracerebroventricular Delivery.
Application : ICC/IF
Cao C et al. Front Microbiol 2019; 10:3040 BICP0 Negatively Regulates TRAF6-Mediated NF-庥B and Interferon Activation by Promoting K48-Linked Polyubiquitination of TRAF6.
Application : WB
Tian JN et al. Viruses 2019; 11 (9) A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.
Application : WB
Li W et al. Cell Stem Cell 2019; 25 (1):54-68.e5 A Homeostatic Arid1a-Dependent Permissive Chromatin State Licenses Hepatocyte Responsiveness to Liver-Injury-Associated YAP Signaling.
Application : ICC/IF
Naik NG et al. Sci Rep 2018; 8 (1):17778 Baculovirus as an efficient vector for gene delivery into mosquitoes.
Application : WB
Hong S et al. Hum Mol Genet 2018; (Epub) Loss-of-function mutations in FGF8 can be independent risk factors for holoprosencephaly.
Application : WB
Tseng TC et al. Biomaterials 2016; 102:148-61 Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair.
Application : ICC/IF, FACS
Yen CS et al. Sci Rep 2016; 6:25082 Lovastatin causes FaDu hypopharyngeal carcinoma cell death via AMPK-p63-survivin signaling cascade.
Application : IP
Cancer 2015; 121 (5):800 Erratum: Borderud SP, Li Y, Burkhalter JE, Sheffer CE and Ostroff JS. Electronic cigarette use among patients with cancer: Characteristics of electronic cigarette users and their smoking cessation outcomes. Cancer. doi: 10.1002/ cncr.28811.
Application : ICC/IF
Huang YH et al. Sci Rep 2015; 5:15900 The effects of a novel aliphatic-chain hydroxamate derivative WMJ-S-001 in HCT116 colorectal cancer cell death.
Application : WB
Lee WY et al. Oncotarget 2015; (Epub) repositioning antipsychotic * for treating colorectal cancer by inhibiting sirtuin 1.
Application : WB, IP
Huang L et al. PLoS One 2014; 9 (3):e92949 HSP40 Interacts with Pyruvate Kinase M2 and Regulates Glycolysis and Cell Proliferation in Tumor Cells.
Application : WB, IP
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169