GeneTex
United States (US)

DDDDK tag antibody

GTX115043_40863_20150306_IFA_18121410_942.jpg
GTX115043_40863_20150410_ELISA_18121410_720.jpg
GTX115043_40863_WB_18121410_542.jpg

Cat No. GTX115043

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

Transfected lysate
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDDDK tag antibody, DDDDK epitope tag antibody, DYKDDDDK epitope tag antibody, DYKDDDDK tag antibody, ECS epitope tag antibody, ECS tag antibody, Enterokinase Cleavage Site epitope tag antibody, Flag tag antibody

Research Area

DATA IMAGES
GTX115043_40863_20150306_IFA_18121410_942.jpg

GTX115043 ICC/IF Image

DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDDDK tag stained by DDDDK tag antibody (GTX115043) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX115043_40863_20150410_ELISA_18121410_720.jpg

GTX115043 ELISA Image

An ELISA plate is coated with 50 μL of a DDDDK tag conjugated BSA at concentration of 10 μg/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody (GTX115043) at concentration ranged from 1.95 to 125 ng/mL.

GTX115043_40863_WB_18121410_542.jpg

GTX115043 WB Image

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing DDDDK-tagged protein (~27 kDa), using DDDDK tag antibody. A: Transfected lysate (10 µg). B: Non-transfected lysate (10 µg). C: Non-transfected lysate (30 µg). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169