ICC/IF analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 1.6 μg/mL(red) with DAPI(blue).

ICC/IF analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 0.8 μg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by acetone.

WB analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only

WB analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only