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DDDDK tag antibody

Anti-DDDDK tag antibody used in Western Blot (WB). GTX73143
Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX73143
Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX73143
Anti-DDDDK tag antibody used in Western Blot (WB). GTX73143

Cat No. GTX73143

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, Dot
Package
100 μg ($329)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2.5 μg/ml
ICC/IF 5-10 μg/ml
IP 4-8 μg
Dot 1-2.5 μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% bovine serum albumin, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

FLAG; peptide sequence DYKDDDDK

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Research Area

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX73143

GTX73143 WB Image

WB analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only

Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX73143

GTX73143 ICC/IF Image

ICC/IF analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 0.8 μg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by acetone.

Anti-DDDDK tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX73143

GTX73143 ICC/IF Image

ICC/IF analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 1.6 μg/mL(red) with DAPI(blue).

Anti-DDDDK tag antibody used in Western Blot (WB). GTX73143

GTX73143 WB Image

WB analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329