APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2.5 μg/ml |
| 5-10 μg/ml |
| 4-8 μg |
| 1-2.5 μg/ml |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
0.01M PBS pH7.4, 1% BSA
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
~0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
FLAG; peptide sequence DYKDDDDK
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
RRID
AB_380231
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Background
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Research Area
DATA IMAGES
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GTX73143 ICC/IF Image
ICC/IF analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 0.8 μg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by acetone.
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GTX73143 ICC/IF Image
ICC/IF analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody at 1.6 μg/mL(red) with DAPI(blue).
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GTX73143 WB Image
WB analysis of HEK-293T cells over-expressing N-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only
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GTX73143 WB Image
WB analysis of HEK-293T cells over-expressing C-Terminal Flag tagged fusion protein using GTX73143 DDDDK tag antibody.
Lane 1 : 0.4 μg/mL antibody
Lane 2 : Secondary antibody only
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REFERENCE
There are currently no references for DDDDK tag antibody (GTX73143). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DDDDK tag antibody (GTX73143). Be the first to share your experience with this product.
