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DDDDK tag antibody

Anti-DDDDK tag antibody used in Western Blot (WB). GTX85472

Cat. No. GTX85472

Reference 2

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Species independent
Package
50 μg ($399)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC 1:500-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10622451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX85472

GTX85472 WB Image

WB analysis of RDF1 transfected cell using GTX85472 DDDDK tag antibody.
Right : Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a DDDDK epitope tag at its C-terminus
Left : Cultured HEK cells with an empty plasmid vector. Loading : 25 ug of protein loaded in each lane.

REFERENCE

Application Reference

Mori A et al. J Biosci Bioeng 2018; (Epub) Zipbodyzyme: Development of new antibody-enzyme fusion proteins.
Application : WB
Ojima-Kato T et al. J Biosci Bioeng 2017; (Epub) N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399