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DDDDK tag antibody

Anti-DDDDK tag antibody used in Western Blot (WB). GTX85472

Cat. No. GTX85472

Reference 2

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IP, ELISA, IHC
Package
50 μg ($399)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC 1:500-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10622451

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX85472

GTX85472 WB Image

WB analysis of RDF1 transfected cell using GTX85472 DDDDK tag antibody.
Right : Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a DDDDK epitope tag at its C-terminus
Left : Cultured HEK cells with an empty plasmid vector. Loading : 25 ug of protein loaded in each lane.

REFERENCE

Application Reference

Mori A et al. J Biosci Bioeng 2018; (Epub) Zipbodyzyme: Development of new antibody-enzyme fusion proteins.
Application : WB
Ojima-Kato T et al. J Biosci Bioeng 2017; (Epub) N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399