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DDDDK tag antibody

Anti-DDDDK tag antibody used in Western Blot (WB). GTX85472

Cat No. GTX85472

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ICC/IF, IP, ELISA, IHC
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC 1:500-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, sodium azide (0.02%, w/v)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-DDDDK tag antibody used in Western Blot (WB). GTX85472

GTX85472 WB Image

WB analysis of RDF1 transfected cell using GTX85472 DDDDK tag antibody.
Right : Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a DDDDK epitope tag at its C-terminus
Left : Cultured HEK cells with an empty plasmid vector. Loading : 25 ug of protein loaded in each lane.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289