DDDDK tag antibody
Host
Chicken
Clonality
Polyclonal
Isotype
IgY
Application
WB, ICC/IF, IP, ELISA, IHC
Package
50 μg ($399 )
Datasheet
Reference
Document
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
1:1000-1:2000
Assay dependent
Assay dependent
Assay dependent
1:500-1:1000
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.2
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_10622451
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX85472 WB Image
WB analysis of RDF1 transfected cell using GTX85472 DDDDK tag antibody.
Right : Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a DDDDK epitope tag at its C-terminus
Left : Cultured HEK cells with an empty plasmid vector.
Loading : 25 ug of protein loaded in each lane.
REFERENCE
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SDS
PBS.pdf
Sodium Azide.pdf
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