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DDI1 antibody [N1C3]

Anti-DDI1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103772
Anti-DDI1 antibody [N1C3] used in Western Blot (WB). GTX103772
Anti-DDI1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103772

Cat. No. GTX103772

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DDI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950104

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage inducible 1 homolog 1

Database

Research Area

DATA IMAGES

Anti-DDI1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103772

GTX103772 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDI1(GTX103772) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDI1 antibody [N1C3] used in Western Blot (WB). GTX103772

GTX103772 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX103772 diluted at 1:1000

Anti-DDI1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103772

GTX103772 ICC/IF Image

DDI1 antibody [N1C3] detects DDI1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDI1 protein stained by DDI1 antibody [N1C3] (GTX103772) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Lee JY et al. Tuberc Respir Dis. 2012; 72:124-131. : 72:124-131. DNA-Damage Inducible 1 is a Property of Human Non-Small Cell Lung Cancer.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169