GeneTex
United States (US)

DDI1 antibody [N1C3]

GTX103772_IHC_19011506_745.jpg
GTX103772_39974_IFA_19011506_395.jpg
GTX103772_WB_19011506_263.jpg

Cat No. GTX103772

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Damage Inducible 1 Homolog 1,Ddi1

Database

DATA IMAGES
GTX103772_IHC_19011506_745.jpg

GTX103772 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDI1(GTX103772) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103772_39974_IFA_19011506_395.jpg

GTX103772 ICC/IF Image

DDI1 antibody [N1C3] detects DDI1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDI1 protein stained by DDI1 antibody [N1C3] (GTX103772) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX103772_WB_19011506_263.jpg

GTX103772 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX103772 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169