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DDR1 antibody [N1N3]

Anti-DDR1 antibody [N1N3] used in Western Blot (WB). GTX111453
Anti-DDR1 antibody [N1N3] used in Western Blot (WB). GTX111453
Anti-DDR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111453
Anti-DDR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111453

Cat. No. GTX111453

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DDR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

Mouse brain , H1299

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human DDR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950106

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

discoidin domain receptor tyrosine kinase 1 , CAK , CD167 , DDR , EDDR1 , HGK2 , MCK10 , NEP , NTRK4 , PTK3 , PTK3A , RTK6 , TRKE

Cellular Localization

Isoform 1: Membrane , Isoform 2: Membrane , Isoform 3: Secreted , Isoform 4: Secreted

Background

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDR1 antibody [N1N3] used in Western Blot (WB). GTX111453

GTX111453 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111453 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR1 antibody [N1N3] used in Western Blot (WB). GTX111453

GTX111453 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111453 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111453

GTX111453 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDR1(GTX111453) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111453

GTX111453 IHC-P Image

DDR1 antibody [N1N3] detects DDR1 protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
DDR1 stained by DDR1 antibody [N1N3] (GTX111453) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Seongki Min et al. Cell Death Dis 2021; 12 (11) : 1076 MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.
Application : WB
Reactivity : Human
Romayor I et al. Am J Physiol Gastrointest Liver Physiol 2021; 320 (6) : G1002-G1013 Tumor DDR1 deficiency reduces liver metastasis by colon carcinoma and impairs stromal reaction.
Okada J et al. Molecules 2020; 25 (3) Dapagliflozin Inhibits Cell Adhesion to Collagen I and IV and Increases Ectodomain Proteolytic Cleavage of DDR1 by Increasing ADAM10 Activity.
C.M. Oefner et al. Placenta 2014; 36 (1) : 59-68 Collagen type IV at the fetal–maternal interface.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169