GeneTex
United States (US)

DDR2 antibody [N2N3]

GTX102526_40387_WB_19011506_563.jpg
GTX102526_40387_IHC_19011506_113.jpg

Cat No. GTX102526

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

K562 , THP-1

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Discoidin Domain Receptor Tyrosine Kinase 2,Mig20A,Ntrkr3,Tkt,Tyro10,Ddr2

Cellular Localization

Membrane

Background

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102526_40387_WB_19011506_563.jpg

GTX102526 WB Image

Sample (30 µg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX102526 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102526_40387_IHC_19011506_113.jpg

GTX102526 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using DDR2(GTX102526) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

DDR2 antibody [N2N3] Cat No. GTX102526

Rating ( Average 3 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 27-Jan-2016
Score :
Application Tested : FACS
Sample Species : Ms
Sample : primary isolated heart cells
Blocking : 1 ug mouse IgG in 1% FBS/ PBS, 25°C, 0.25Hr
Primary Antibody : 1:100, 25°C, 0.6Hr
Figure Note : Orange area: CD31 expression endothelial cells, Blue area: DDR2 expression cardiac fibroblasts (GTX102526), Green area: Double negative cells
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169