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DDT antibody [N1C3]

Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791
Anti-DDT antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101791
Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791
Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

Cat No. GTX101791

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T , HeLa , Raji , mouse liver , rat liver

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

D-dopachrome tautomerase , D-DT , DDCT , MIF-2 , MIF2

Cellular Localization

Cytoplasm

Background

D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

GTX101791 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
GTX101791 diluted at 1:500

Anti-DDT antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101791

GTX101791 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DDT(GTX101791) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

GTX101791 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] (GTX101791) diluted at 1:500.

Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

GTX101791 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] (GTX101791) diluted at 1:500.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169