GeneTex
United States (US)

DDT antibody [N1C3]

GTX101791_WB_19011506_531.jpg
GTX101791_IHC_19011506_513.jpg
GTX101791_40121_20160107_WB_R_liver_19011506_233.jpg
GTX101791_40121_20160107_WB_M_liver_19011506_107.jpg

Cat No. GTX101791

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T , HeLa , Raji , mouse liver , rat liver

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDCT antibody, DDT antibody, phenylpyruvate tautomerase II antibody, D-dopachrome decarboxylase antibody, D-dopachrome tautomerase antibody

Cellular Localization

Cytoplasm

Background

D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq]

Database

DATA IMAGES
GTX101791_WB_19011506_531.jpg

GTX101791 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
GTX101791 diluted at 1:500

GTX101791_IHC_19011506_513.jpg

GTX101791 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DDT(GTX101791) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101791_40121_20160107_WB_R_liver_19011506_233.jpg

GTX101791 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] (GTX101791) diluted at 1:500.

GTX101791_40121_20160107_WB_M_liver_19011506_107.jpg

GTX101791 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] (GTX101791) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169