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DDX1 antibody [N3C2], Internal

KO/KD
Anti-DDX1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105205
Anti-DDX1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105205
Anti-DDX1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105205
Anti-DDX1 antibody [N3C2], Internal used in Western Blot (WB). GTX105205

Cat. No. GTX105205

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)
See all DDX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431 , H1299

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036748

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 1 , DBP-RB , UKVH5d

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDX1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105205

GTX105205 IHC-P Image

DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
DDX1 antibody [N3C2], Internal (GTX105205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDX1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105205

GTX105205 IHC-P Image

DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
DDX1 antibody [N3C2], Internal (GTX105205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDX1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105205

GTX105205 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DDX1(GTX105205) antibody at 1:200 dilution.

Anti-DDX1 antibody [N3C2], Internal used in Western Blot (WB). GTX105205

GTX105205 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX1 antibody [N3C2], Internal (GTX105205) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yasuo Ariumi et al. J Virol 2022; 96 (6) : e0000222 Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection.
Application : WB
Reactivity : Monkey
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169