GeneTex
United States (US)

DDX1 antibody [N3C2], Internal

KO/KD
GTX105205_39904_IHC_R_19011506_805.jpg
GTX105205_39904_IHC_M_19011506_484.jpg
GTX105205_WB_19011506_407.jpg
GTX105205_39904_IFA_19011506_200.jpg

Cat No. GTX105205

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DBP-RB antibody, UKVH5d antibody, DDX1 antibody, ATP-dependent RNA helicase DDX1 antibody, DEAD box protein retinoblastoma antibody, DEAD box protein 1 antibody, DEAD box polypeptide 1 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1 antibody, DEAD box-1 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 antibody

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq]
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Database

DATA IMAGES
GTX105205_39904_IHC_R_19011506_805.jpg

GTX105205 IHC-P Image

DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
DDX1 antibody [N3C2], Internal (GTX105205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105205_39904_IHC_M_19011506_484.jpg

GTX105205 IHC-P Image

DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
DDX1 antibody [N3C2], Internal (GTX105205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105205_WB_19011506_407.jpg

GTX105205 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX105205 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105205_39904_IFA_19011506_200.jpg

GTX105205 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DDX1(GTX105205) antibody at 1:200 dilution.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169