APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:10 - 1:100 |
| 1:50 - 1:200 |
| 1:20 - 1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DEAD-box helicase 1 , DBP-RB , UKVH5d
Cellular Localization
Nucleus,Cytoplasm,Cytoplasm, cytosol,Mitochondrion
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX33149 WB Image
WB analysis of various sample lysates using GTX33149 DDX1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX33149 IHC-P Image
IHC-P analysis of rat brain tissue using GTX33149 DDX1 antibody.
Dilution : 1:100
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GTX33149 IHC-P Image
IHC-P analysis of human leiomyoma of uterus tissue using GTX33149 DDX1 antibody.
Dilution : 1:100
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GTX33149 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX33149 DDX1 antibody.
Dilution : 1:100
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GTX33149 IP Image
IP analysis of 293T cell lysate using GTX33149 DDX1 antibody.
Antibody amount : 3μg / 100μg lysate
Dilution : 1:1000
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GTX33149 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX33149 DDX1 antibody.
Dilution : 1:100
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GTX33149 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX33149 DDX1 antibody.
Blue : DAPI
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REFERENCE
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REVIEW
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