GeneTex
United States (US)

DDX1 antibody

GTX33149_20180530_IHC-P_1_18121410_971.jpg
GTX33149_20180530_ICCIF_18121410_931.jpg
GTX33149_20180530_IHC-P_2_18121410_139.jpg
GTX33149_20180530_IHC-P_3_18121410_616.jpg
GTX33149_20180530_WB_18121410_781.jpg
GTX33149_20180530_IP_18121410_210.jpg
GTX33149_20180530_IHC-P_18121410_129.jpg

Cat No. GTX33149

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dead-Box Helicase 1,Dbp-Rb,Ukvh5D,Ddx1

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX33149_20180530_IHC-P_1_18121410_971.jpg

GTX33149 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33149 DDX1 antibody.
Dilution : 1:100

GTX33149_20180530_ICCIF_18121410_931.jpg

GTX33149 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33149 DDX1 antibody.
Blue : DAPI

GTX33149_20180530_IHC-P_2_18121410_139.jpg

GTX33149 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX33149 DDX1 antibody.
Dilution : 1:100

GTX33149_20180530_IHC-P_3_18121410_616.jpg

GTX33149 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33149 DDX1 antibody.
Dilution : 1:100

GTX33149_20180530_WB_18121410_781.jpg

GTX33149 WB Image

WB analysis of various samples using GTX33149 DDX1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX33149_20180530_IP_18121410_210.jpg

GTX33149 IP Image

IP analysis of 293T cell lysate using GTX33149 DDX1 antibody.
IP reaction : 3μg antibody / 100μg cell lysate
Dilution : 1:1000

GTX33149_20180530_IHC-P_18121410_129.jpg

GTX33149 IHC-P Image

IHC-P analysis of human uterus leiomyoma tissue using GTX33149 DDX1 antibody.
Dilution : 1:100

Package List Price ($)
$ 329