APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
Observed MW
72|82 kDa. The observed M.W. is based on the following publication. PMID: 31653828
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human DDX17
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DEAD-box helicase 17 , P72 , RH70
Cellular Localization
Nucleus , Cytoplasm, cytosol , Nucleolus
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]
Database
Research Area
DATA IMAGES
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/templates/default/js/lightbox/images/loading.gif "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
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GTX02851 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX17 antibody [GT1254] (GTX02851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 31653828
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GTX02851 IHC-P Image
IHC-P analysis of mouse spinal cord section using GTX02851 DDX17 antibody [GT1254].
Dilution : 1:100
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GTX02851 IHC-P Image
IHC-P analysis of rat testis tissue section using GTX02851 DDX17 antibody [GT1254].
Dilution : 1:100
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GTX02851 WB Image
WB analysis of various samples using GTX02851 DDX17 antibody [GT1254].
Dilution : 1:1000
Loading : 25μg
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GTX02851 WB Image
WB analysis of various samples using GTX02851 DDX17 antibody [GT1254].
Dilution : 1:1000
Loading : 25μg
|
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GTX02851 IHC-P Image
IHC-P analysis of human colon tissue section using GTX02851 DDX17 antibody [GT1254].
Dilution : 1:100
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REFERENCE
There are currently no references for DDX17 antibody [GT1254] (GTX02851). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DDX17 antibody [GT1254] (GTX02851). Be the first to share your experience with this product.
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/upload/website/prouct_img/small/GTX02851/GTX02851_4000001413_20210122_WB_w_23053123_887.webp "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
![Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851](/upload/website/prouct_img/small/GTX02851/A5731_IHC_03_(1072097)_w_23053123_753.webp "Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851")
![Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851](/upload/website/prouct_img/small/GTX02851/A5731_IHC_01_(1072095)_w_23053123_466.webp "Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851")
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/upload/website/prouct_img/small/GTX02851/CutImage_A5731_WB_02_(1071162)_w_23053123_951.webp "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/upload/website/prouct_img/small/GTX02851/CutImage_A5731_WB_01_(1071161)_w_23053123_102.webp "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
![Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851](/upload/website/prouct_img/small/GTX02851/A5731_IHC_02_(1072096)_w_23053123_767.webp "Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851")
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/upload/website/prouct_img/normal/GTX02851/GTX02851_4000001413_20210122_WB_w_23053123_887.webp "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
![Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851](/upload/website/prouct_img/normal/GTX02851/A5731_IHC_03_(1072097)_w_23053123_753.webp "Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851")
![Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851](/upload/website/prouct_img/normal/GTX02851/A5731_IHC_01_(1072095)_w_23053123_466.webp "Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851")
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/upload/website/prouct_img/normal/GTX02851/CutImage_A5731_WB_02_(1071162)_w_23053123_951.webp "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
![Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851](/upload/website/prouct_img/normal/GTX02851/CutImage_A5731_WB_01_(1071161)_w_23053123_102.webp "Anti-DDX17 antibody [GT1254] used in Western Blot (WB). GTX02851")
![Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851](/upload/website/prouct_img/normal/GTX02851/A5731_IHC_02_(1072096)_w_23053123_767.webp "Anti-DDX17 antibody [GT1254] used in IHC (Paraffin sections) (IHC-P). GTX02851")