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DDX17 antibody

Anti-DDX17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02534
Anti-DDX17 antibody used in Western Blot (WB). GTX02534
Anti-DDX17 antibody used in Western Blot (WB). GTX02534

Cat No. GTX02534

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DDX17, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEAD-box helicase 17 , P72 , RH70

Cellular Localization

Nucleus , Cytoplasm, cytosol , Nucleolus

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-DDX17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02534

GTX02534 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02534 DDX17 Antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-DDX17 antibody used in Western Blot (WB). GTX02534

GTX02534 WB Image

WB analysis of extracts from HEK293 cells using GTX02534 DDX17 Antibody. The lane on the left was treated with blocking peptide.

Anti-DDX17 antibody used in Western Blot (WB). GTX02534

GTX02534 WB Image

WB analysis of extracts from HeLa cells using GTX02534 DDX17 Antibody. The lane on the left was treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 339