APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:2000 |
~1:500 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified Human DDX18 fused with GST
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DEAD-box helicase 18 , Has1 , MrDb
Cellular Localization
Nucleolus
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00725 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00725 DDX18 antibody. Green : Primary antibody Blue : Hoechst 33342
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GTX00725 WB Image
WB analysis of HeLa whole cell lysate using GTX00725 DDX18 antibody. Dilution : 1:1000 Loading : 10μg
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REFERENCE
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REVIEW
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