APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:20000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HepG2
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.02 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX19B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10621848
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DEAD-box helicase 19B , DBP5 , DDX19 , RNAh
Cellular Localization
Cytoplasm , Nucleus , nuclear pore complex , Nucleus membrane
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115612 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX19B(GTX115612) antibody at 1:200 dilution.
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GTX115612 WB Image
Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 (GTX27900) 10% SDS PAGE GTX115612 diluted at 1:10000
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REFERENCE
There are currently no references for DDX19B antibody [N1N3] (GTX115612). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DDX19B antibody [N1N3] (GTX115612). Be the first to share your experience with this product.