*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , HepG2 , HCT116
Mouse, Rat, Sheep, Bovine(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human DDX21. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Dexd-Box Helicase 21,Gua,Gurdb,Rh-Ii/Gu,Rh-Ii/Gua,Ddx21
Nucleus , nucleolus
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq]
GTX115199 ICC/IF Image
DDX21 antibody [N1N3] detects DDX21 protein at nucleolus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX21 protein stained by DDX21 antibody [N1N3] (GTX115199) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX115199 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX115199 diluted at 1:1000