APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HepG2
Predict Reactivity
Mouse, Rat, Sheep, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX21. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11164504
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DExD-box helicase 21 , GUA , GURDB , RH-II/GU , RH-II/GuA
Cellular Localization
Nucleus , nucleolus
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX115199 ICC/IF Image
DDX21 antibody [N1N3] detects DDX21 protein at nucleolus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX21 protein stained by DDX21 antibody [N1N3] (GTX115199) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
|
|
GTX115199 WB Image
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE GTX115199 diluted at 1:1000
|
REFERENCE
There are currently no references for DDX21 antibody [N1N3] (GTX115199). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DDX21 antibody [N1N3] (GTX115199). Be the first to share your experience with this product.