GeneTex
United States (US)

DDX23 antibody

GTX115234_40254_20141225_IFA_18121410_625.jpg
GTX115234_40254_WB_M_18121410_488.jpg
GTX115234_WB_18121410_263.jpg
GTX115234_40254_IHC_18121410_359.jpg

Cat No. GTX115234

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

Jurkat , NIH-3T3 , JC

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dead-Box Helicase 23,Prpf28,Snrnp100,U5-100K,U5-100Kd,Prp28,Ddx23

Cellular Localization

Nucleus

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq]

Database

DATA IMAGES
GTX115234_40254_20141225_IFA_18121410_625.jpg

GTX115234 ICC/IF Image

DDX23 antibody detects DDX23 protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX23 protein stained by DDX23 antibody (GTX115234) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX115234_40254_WB_M_18121410_488.jpg

GTX115234 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX115234 diluted at 1:1000

GTX115234_WB_18121410_263.jpg

GTX115234 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX115234 diluted at 1:1000

GTX115234_40254_IHC_18121410_359.jpg

GTX115234 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AZ521 xenograft, using DDX23(GTX115234) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169