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DDX28 antibody [N2C1], Internal

Anti-DDX28 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX116603
Anti-DDX28 antibody [N2C1], Internal used in Western Blot (WB). GTX116603

Cat No. GTX116603

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX28. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617398

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEAD-box helicase 28 , MDDX28

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDX28 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX116603

GTX116603 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DDX28(GTX116603) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX28 antibody [N2C1], Internal used in Western Blot (WB). GTX116603

GTX116603 WB Image

Sample (30 ug of whole cell lysate)
A:IMR32
7.5% SDS PAGE
GTX116603 diluted at 1:1000

REFERENCE

There are currently no references for DDX28 antibody [N2C1], Internal (GTX116603). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DDX28 antibody [N2C1], Internal (GTX116603). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169