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DDX3 antibody [N3C2], Internal

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

Cat No. GTX110614

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , U87MG , U87-MG Cytoplasm , Human ESC

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036751

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , DEAD-box helicase 3 Y-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102 , DBY

Cellular Localization

Cytoplasm , Nucleus , Nucleus speckle , Mitochondrion outer membrane

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000.

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX110614 diluted at 1:2000

Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614

GTX110614 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DDX3(GTX110614) antibody at 1:200 dilution.

Anti-DDX3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110614

GTX110614 IHC-P Image

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614

GTX110614 ICC/IF Image

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169