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DDX3 antibody [N3C2], Internal

Orthogonal
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110614
Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

Cat. No. GTX110614

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DDX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

Human ESC , U87MG

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036751

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , DEAD-box helicase 3 Y-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102 , DBY

Cellular Localization

Cytoplasm , Nucleus , Nucleus speckle , Mitochondrion outer membrane

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614

GTX110614 ICC/IF Image

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-DDX3 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110614

GTX110614 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DDX3(GTX110614) antibody at 1:200 dilution.

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Orthogonal

U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000.

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX110614 diluted at 1:2000

Anti-DDX3 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110614

GTX110614 IHC-P Image

DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX3 antibody [N3C2], Internal used in Western Blot (WB). GTX110614

GTX110614 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Sarah C Atkinson et al. Int J Mol Sci 2021; 22 (16) TRIM25 and DEAD-Box RNA Helicase DDX3X Cooperate to Regulate RIG-I-Mediated Antiviral Immunity.
Application : WB
Reactivity : Human
Deschepper CF et al. Sci Rep 2020; 10 (1) : 14900 Regulatory effects of the Uty/Ddx3y locus on neighboring chromosome Y genes and autosomal mRNA transcripts in adult mouse non-reproductive cells.
Application : WB
Reactivity : Mouse
Tsai WC et al. Appl Immunohistochem Mol Morphol 2018; 26 (4) : 263-267 Applying DDX3X Biomarker to Discriminate Atypical from Benign Meningiomas in Tissue Microarray.
Application : IHC-P
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169