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DDX3 antibody

Orthogonal
Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00724
Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00724
Anti-DDX3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00724
Anti-DDX3 antibody used in Western Blot (WB). GTX00724

Cat. No. GTX00724

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Monkey, Rodent
Package
100 μl ($399)
See all DDX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:500
IHC-Fr 1:200
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified full-lenght human DDX3X fused with GST

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102

Cellular Localization

Nucleus speckle,Cytoplasm,Mitochondrion outer membrane

Background

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00724

GTX00724 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells treated with/without LMB using GTX00724 DDX3 antibody.
LMB : a nuclear translocation inhibitor
Dilution : 1:500
Fixation : 4% PFA
DDX3X is localized in cytoplasm in the absence of treatment, but once treated with LMB, a nuclear translocation inhibitor, it is localized not only in cytoplasm but also in nuclei.

Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00724

GTX00724 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00724 DDX3 antibody.
Red : Primary antibody
Blue : Hoechst 33342
Dilution : 1:500

Anti-DDX3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00724

GTX00724 IHC-Fr Image

IHC-Fr analysis of mouse testis tissue section using GTX00724 DDX3 antibody.
Arrows indicate Leydig cells.
Dilution : 1:200

Anti-DDX3 antibody used in Western Blot (WB). GTX00724

GTX00724 WB Image

WB analysis of HeLa whole cell lysate using GTX00724 DDX3 antibody.
Dilution : 1:1000
Loading : 10μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399