WB analysis of HeLa whole cell lysate using GTX00724 DDX3 antibody.
Dilution : 1:1000
Loading : 10μg

IHC-Fr analysis of mouse testis tissue section using GTX00724 DDX3 antibody.
Arrows indicate Leydig cells.
Dilution : 1:200

ICC/IF analysis of HeLa cells using GTX00724 DDX3 antibody.
Red : Primary antibody
Blue : Hoechst 33342
Dilution : 1:500

ICC/IF analysis of HeLa cells treated with/without LMB using GTX00724 DDX3 antibody.
LMB : a nuclear translocation inhibitor
Dilution : 1:500
Fixation : 4% PFA
DDX3X is localized in cytoplasm in the absence of treatment, but once treated with LMB, a nuclear translocation inhibitor, it is localized not only in cytoplasm but also in nuclei.