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DDX3 antibody

Anti-DDX3 antibody used in Western Blot (WB). GTX109727
Anti-DDX3 antibody used in Western Blot (WB). GTX109727
Anti-DDX3 antibody used in Western Blot (WB). GTX109727
Anti-DDX3 antibody used in Western Blot (WB). GTX109727
Anti-DDX3 antibody used in Western Blot (WB). GTX109727
Anti-DDX3 antibody used in Western Blot (WB). GTX109727
Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109727

Cat. No. GTX109727

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DDX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

H1299 , HepG2 , Mouse brain

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950108

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , DEAD-box helicase 3 Y-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102 , DBY

Cellular Localization

Cytoplasm , Nucleus , Nucleus speckle , Mitochondrion outer membrane

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody used in Western Blot (WB). GTX109727

GTX109727 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (GTX109727) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDX3 antibody used in Western Blot (WB). GTX109727

GTX109727 WB Image

DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 50 μg rat testis lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (GTX109727) dilution: 1:2000

Anti-DDX3 antibody used in Western Blot (WB). GTX109727

GTX109727 WB Image

DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (GTX109727) dilution: 1:5000

Anti-DDX3 antibody used in Western Blot (WB). GTX109727

GTX109727 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (GTX109727) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDX3 antibody used in Western Blot (WB). GTX109727

GTX109727 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX109727 diluted at 1:1000

Anti-DDX3 antibody used in Western Blot (WB). GTX109727

GTX109727 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX109727 diluted at 1:10000

Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109727

GTX109727 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3 (GTX109727) antibody at 1:200 dilution.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169