Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3 (GTX109727) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX109727 diluted at 1:10000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX109727 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (GTX109727) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (GTX109727) dilution: 1:5000

DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 50 μg rat testis lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (GTX109727) dilution: 1:2000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (GTX109727) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.