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DDX3 antibody

Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114360
Anti-DDX3 antibody used in Western Blot (WB). GTX114360

Cat. No. GTX114360

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163661

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 3 X-linked , DEAD-box helicase 3 Y-linked , CAP-Rf , DBX , DDX14 , DDX3 , HLP2 , MRX102 , DBY

Cellular Localization

Cytoplasm , Nucleus , Nucleus speckle , Mitochondrion outer membrane

Database

Research Area

DATA IMAGES

Anti-DDX3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114360

GTX114360 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3(GTX114360) antibody at 1:500 dilution.

Anti-DDX3 antibody used in Western Blot (WB). GTX114360

GTX114360 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (GTX114360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169