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DDX3Y antibody

Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004
Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004
Anti-DDX3Y antibody used in Western Blot (WB). GTX66004
Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004
Anti-DDX3Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66004
Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004
Anti-DDX3Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66004
Anti-DDX3Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66004

Cat. No. GTX66004

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DDX3Y products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 3 Y-linked , DBY

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004

GTX66004 IHC-P Image

IHC-P analysis of rat testis tissue using GTX66004 DDX3Y antibody.
Dilution : 1:100

Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004

GTX66004 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX66004 DDX3Y antibody.
Dilution : 1:100

Anti-DDX3Y antibody used in Western Blot (WB). GTX66004

GTX66004 WB Image

WB analysis of various sample lysates using GTX66004 DDX3Y antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004

GTX66004 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX66004 DDX3Y antibody.
Dilution : 1:100

Anti-DDX3Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66004

GTX66004 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66004 DDX3Y antibody.
Blue : DAPI
Dilution : 1:100

Anti-DDX3Y antibody used in IHC (Paraffin sections) (IHC-P). GTX66004

GTX66004 IHC-P Image

IHC-P analysis of rat brain tissue using GTX66004 DDX3Y antibody.
Dilution : 1:100

Anti-DDX3Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66004

GTX66004 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66004 DDX3Y antibody.
Blue : DAPI
Dilution : 1:100

Anti-DDX3Y antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66004

GTX66004 ICC/IF Image

ICC/IF analysis of C6 cells using GTX66004 DDX3Y antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399