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DDX4 antibody [2F9H5]

Anti-DDX4 antibody [2F9H5] used in Western Blot (WB). GTX83289
Anti-DDX4 antibody [2F9H5] used in Flow cytometry (FACS). GTX83289
Anti-DDX4 antibody [2F9H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83289
Anti-DDX4 antibody [2F9H5] used in IHC (Paraffin sections) (IHC-P). GTX83289

Cat. No. GTX83289

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F9H5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all DDX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human DDX4 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 4 , VASA

Cellular Localization

Cytoplasm

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-DDX4 antibody [2F9H5] used in Western Blot (WB). GTX83289

GTX83289 WB Image

WB analysis of DDX4-hIgGFc transfected HEK293 cell lysate using GTX83289 DDX4 antibody [2F9H5].

Anti-DDX4 antibody [2F9H5] used in Flow cytometry (FACS). GTX83289

GTX83289 FACS Image

FACS analysis of MSCS cells using GTX83289 DDX4 antibody [2F9H5].
Green : DDX4
Purple : negative control

Anti-DDX4 antibody [2F9H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83289

GTX83289 ICC/IF Image

ICC/IF analysis of MSCs(left) and NTERA-2 (right) cells using GTX83289 DDX4 antibody [2F9H5].
Green : DDX4
Red: Actin filaments

Anti-DDX4 antibody [2F9H5] used in IHC (Paraffin sections) (IHC-P). GTX83289

GTX83289 IHC-P Image

IHC-P analysis of human lung cancer (A) and rectal cancer (B) using GTX83289 DDX4 antibody [2F9H5].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399