DDX4 antibody [C1C3]

Cat. No. GTX116575

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm
Reactivity	Human, Mouse, Siberian sturgeon, Fish
Package	100 μl ($399), 25 μl ($169)

See all DDX4 products

View product citations for antibody GTX116575 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618817

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 4 , VASA

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq]

Database

Gene ID: 54514 DDX4
UniProt: Q9NQI0 DDX4

Research Area

DATA IMAGES

GTX116575 WB Image

Mouse tissue extract (30 | 50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [C1C3] (GTX116575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116575 WB Image

Human tissue extracts (30 | 50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [C1C3] (GTX116575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116575 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX116575 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116575 IHC-P Image

DDX4 antibody [C1C3] detects DDX4 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DDX4 antibody [C1C3] (GTX116575) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min