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DDX4 antibody [HL1487]

Recombinant antibody Orthogonal
Anti-DDX4 antibody [HL1487] used in IHC (Paraffin sections) (IHC-P). GTX636961
Anti-DDX4 antibody [HL1487] used in IHC (Paraffin sections) (IHC-P). GTX636961
Anti-DDX4 antibody [HL1487] used in IHC (Paraffin sections) (IHC-P). GTX636961
Anti-DDX4 antibody [HL1487] used in Western Blot (WB). GTX636961
Anti-DDX4 antibody [HL1487] used in Western Blot (WB). GTX636961
Anti-DDX4 antibody [HL1487] used in Western Blot (WB). GTX636961

Cat. No. GTX636961

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1487

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DDX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 4 , VASA

Cellular Localization

Cytoplasm

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-DDX4 antibody [HL1487] used in IHC (Paraffin sections) (IHC-P). GTX636961

GTX636961 IHC-P Image

DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX4 antibody [HL1487] used in IHC (Paraffin sections) (IHC-P). GTX636961

GTX636961 IHC-P Image

DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX4 antibody [HL1487] used in IHC (Paraffin sections) (IHC-P). GTX636961

GTX636961 IHC-P Image

DDX4 antibody [HL1487] detects DDX4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DDX4 antibody [HL1487] used in Western Blot (WB). GTX636961

GTX636961 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDX4 antibody [HL1487] used in Western Blot (WB). GTX636961

GTX636961 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDX4 antibody [HL1487] used in Western Blot (WB). GTX636961

GTX636961 WB Image

Human tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations