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DDX5 antibody, C-term

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261
Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261
Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261
Anti-DDX5 antibody, C-term used in Western Blot (WB). GTX10261

Cat No. GTX10261

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10261-PEP).

Predict Reactivity

Rat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dead-Box Helicase 5 , G17P1 , Hlr1 , Hump68 , P68 , Ddx5

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

GTX10261 IHC-P Image

IHC-P analysis of human uterus using GTX10261 DDX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

GTX10261 IHC-P Image

IHC-P analysis of human breast using GTX10261 DDX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

GTX10261 IHC-P Image

IHC-P analysis of human prostate using GTX10261 DDX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Anti-DDX5 antibody, C-term used in Western Blot (WB). GTX10261

GTX10261 WB Image

WB analysis of NIH-3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates using GTX10261 DDX5 antibody, C-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

REFERENCE
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REVIEW
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Package List Price ($)
$ 329