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DDX5 antibody, C-term

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261
Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261
Anti-DDX5 antibody, C-term used in Western Blot (WB). GTX10261
Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

Cat. No. GTX10261

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all DDX5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10261-PEP).

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 5 , G17P1 , HLR1 , HUMP68 , p68

Cellular Localization

Nucleolus

Background

This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

GTX10261 IHC-P Image

IHC-P analysis of human uterus using GTX10261 DDX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

GTX10261 IHC-P Image

IHC-P analysis of human prostate using GTX10261 DDX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-DDX5 antibody, C-term used in Western Blot (WB). GTX10261

GTX10261 WB Image

WB analysis of NIH-3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates using GTX10261 DDX5 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-DDX5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10261

GTX10261 IHC-P Image

IHC-P analysis of human breast using GTX10261 DDX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399