GeneTex
United States (US)

DDX5 antibody [C3], C-term

GTX100234_39274_IFA_19011506_142.jpg
GTX100234_WB_19011506_812.jpg
GTX100234_IHC_19011506_605.jpg

Cat No. GTX100234

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dead-Box Helicase 5,G17P1,Hlr1,Hump68,P68,Ddx5

Cellular Localization

Nucleus , nucleolus

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100234_39274_IFA_19011506_142.jpg

GTX100234 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using DDX5(GTX100234) antibody at 1:500 dilution.

GTX100234_WB_19011506_812.jpg

GTX100234 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
DDX5 antibody
GTX100234 diluted at 1:1000

GTX100234_IHC_19011506_605.jpg

GTX100234 IHC-P Image

Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using DDX5(GTX100234) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf