GeneTex

  • Country / Location Selection

United States (US)

DDX5 antibody [C3], C-term

Anti-DDX5 antibody [C3], C-term used in Western Blot (WB). GTX100234
Anti-DDX5 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100234
Anti-DDX5 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100234

Cat. No. GTX100234

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DDX5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240694

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 5 , G17P1 , HLR1 , HUMP68 , p68

Cellular Localization

Nucleus , nucleolus

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDX5 antibody [C3], C-term used in Western Blot (WB). GTX100234

GTX100234 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
DDX5 antibody
GTX100234 diluted at 1:1000

Anti-DDX5 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100234

GTX100234 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using DDX5(GTX100234) antibody at 1:500 dilution.

Anti-DDX5 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100234

GTX100234 IHC-P Image

Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using DDX5(GTX100234) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yang TW et al. J Proteome Res 2018; (Epub) RNA-binding proteomics reveals MATR3 interacting with lncRNA SNHG1 to enhance neuroblastoma progression.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF, IP
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for DDX5 antibody [C3], C-term (GTX100234). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169