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DDX52 antibody

Anti-DDX52 antibody used in Western Blot (WB). GTX120797
Anti-DDX52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120797

Cat. No. GTX120797

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX52. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885529

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DExD-box helicase 52 , HUSSY19 , ROK1

Cellular Localization

Nucleus , nucleolus

Database

Research Area

DATA IMAGES

Anti-DDX52 antibody used in Western Blot (WB). GTX120797

GTX120797 WB Image

DDX52 antibody detects DDX52 protein by Western blot analysis.
A. 30 μg Raji whole cell lysate/extract
7.5 % SDS-PAGE
DDX52 antibody (GTX120797) dilution: 1:1000

Anti-DDX52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120797

GTX120797 ICC/IF Image

DDX52 antibody detects DDX52 protein at nucleolus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: DDX52 protein stained by DDX52 antibody (GTX120797) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169