GeneTex
United States (US)

DDX56 antibody

GTX115551_40212_20150612_IHC_M_18121410_743.jpg
GTX115551_WB_18121410_605.jpg
GTX115551_40212_IFA_18121410_522.jpg
GTX115551_40212_IHC_18121410_938.jpg

Cat No. GTX115551

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX56. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dead-Box Helicase 56,Ddx21,Ddx26,Noh61,Ddx56

Cellular Localization

Nucleus , nucleolus

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. [provided by RefSeq]

Database

DATA IMAGES
GTX115551_40212_20150612_IHC_M_18121410_743.jpg

GTX115551 IHC-P Image

DDX56 antibody detects DDX56 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DDX56 antibody (GTX115551) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115551_WB_18121410_605.jpg

GTX115551 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX115551 diluted at 1:1000

GTX115551_40212_IFA_18121410_522.jpg

GTX115551 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DDX56(GTX115551) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115551_40212_IHC_18121410_938.jpg

GTX115551 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using DDX56(GTX115551) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169