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DDX6 antibody

Anti-DDX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102795
Anti-DDX6 antibody used in Western Blot (WB). GTX102795
Anti-DDX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX102795

Cat No. GTX102795

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Guinea pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.708 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEAD-box helicase 6 , HLR2 , P54 , RCK

Cellular Localization

Cytoplasm , P-body

Background

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq]

Database

DATA IMAGES

Anti-DDX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102795

GTX102795 ICC/IF Image

DDX6 antibody detects DDX6 protein at cytoplasm and P body by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX6 protein stained by DDX6 antibody (GTX102795) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-DDX6 antibody used in Western Blot (WB). GTX102795

GTX102795 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
DDX6 antibody
GTX102795 diluted at 1:1000

Anti-DDX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX102795

GTX102795 IHC-P Image

DDX6 antibody detects DDX6 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
DDX6 antibody (GTX102795) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169