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DDX6 antibody

Anti-DDX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX102795
Anti-DDX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102795
Anti-DDX6 antibody used in Western Blot (WB). GTX102795

Cat. No. GTX102795

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DDX6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Guinea pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240695

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD-box helicase 6 , HLR2 , P54 , RCK

Cellular Localization

Cytoplasm , P-body

Background

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDX6 antibody used in IHC (Paraffin sections) (IHC-P). GTX102795

GTX102795 IHC-P Image

DDX6 antibody detects DDX6 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
DDX6 antibody (GTX102795) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102795

GTX102795 ICC/IF Image

DDX6 antibody detects DDX6 protein at cytoplasm and P body by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX6 protein stained by DDX6 antibody (GTX102795) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DDX6 antibody used in Western Blot (WB). GTX102795

GTX102795 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
DDX6 antibody
GTX102795 diluted at 1:1000

REFERENCE

Application Reference

Arora R et al. EMBO Rep 2022; 23 (9) : e54458 Sequestration of LINE-1 in cytosolic aggregates by MOV10 restricts retrotransposition.
Application : WB, ICC/IF
Reactivity : Mouse
Jeremiah J Bearss et al. EMBO Rep 2021; 22 (4) : e50835 EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics.
Application : WB
Reactivity : Human
Maeda R et al. Cell Death Dis 2021; 12 (4) : 285 RNA decay in processing bodies is indispensable for adipogenesis.
Application : WB
Reactivity : Mouse
Duan Z et al. Biosci Rep 2018; (Epub) Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells.
Application : IHC-P
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169