GeneTex
United States (US)

DDX6 antibody

GTX102795_39694_IFA_19011506_102.jpg
GTX102795_WB_19011506_908.jpg
GTX102795_39694_20141114_IHC_M_19011506_246.jpg

Cat No. GTX102795

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Guinea pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.708 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dead-Box Helicase 6,Hlr2,P54,Rck,Ddx6

Cellular Localization

Cytoplasm , P-body

Background

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq]

Database

DATA IMAGES
GTX102795_39694_IFA_19011506_102.jpg

GTX102795 ICC/IF Image

DDX6 antibody detects DDX6 protein at cytoplasm and P body by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DDX6 protein stained by DDX6 antibody (GTX102795) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102795_WB_19011506_908.jpg

GTX102795 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
DDX6 antibody
GTX102795 diluted at 1:1000

GTX102795_39694_20141114_IHC_M_19011506_246.jpg

GTX102795 IHC-P Image

DDX6 antibody detects DDX6 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
DDX6 antibody (GTX102795) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169