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DDX60 antibody

KO/KD
Anti-DDX60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32082
Anti-DDX60 antibody used in Western Blot (WB). GTX32082

Cat. No. GTX32082

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all DDX60 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Positive Control

A-20 Cell Slide(GTX25541) , A20 Cell Lysate(GTX27180)

Product Note

At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.The immunogen is located within amino acids 1140 - 1190 of DDX60.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DExD/H-box helicase 60

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-DDX60 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32082

GTX32082 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX32082 DDX60 antibody.
Working concentration : 20 μg/ml

Anti-DDX60 antibody used in Western Blot (WB). GTX32082

GTX32082 WB Image

WB analysis of A20 cell lysate using GTX32082 DDX60 antibody.
Dilution : 1μg/ml (Lane A) and 2 μg/ml (Lane B)

REFERENCE

Application Reference

Tiantian Lai et al. Biochem. Biophys. Rep. 2023; 34 : 101488 The DEAD-box RNA helicase, DDX60, Suppresses immunotherapy and promotes malignant progression of pancreatic cancer
Application : WB
Reactivity : Human
Takao Karasawa et al. Ren Fail 2022; 44 (1) : 137-145 Glomerular endothelial expression of type I IFN-stimulated gene, DExD/H-Box helicase 60 via toll-like receptor 3 signaling: possible involvement in the pathogenesis of lupus nephritis.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399