GeneTex

  • Country / Location Selection

United States (US)

DECR1 antibody [N1C3]

Anti-DECR1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109608
Anti-DECR1 antibody [N1C3] used in Western Blot (WB). GTX109608
Anti-DECR1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109608
Anti-DECR1 antibody [N1C3] used in Western Blot (WB). GTX109608
Anti-DECR1 antibody [N1C3] used in Western Blot (WB). GTX109608

Cat. No. GTX109608

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DECR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse liver , HepG2 , Rat heart

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human DECR1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950110

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2,4-dienoyl-CoA reductase 1 , DECR , NADPH , SDR18C1

Cellular Localization

Mitochondrion

Background

This gene encodes an accessory enzyme which participates in the beta-oxidation and metabolism of unsaturated fatty enoyl-CoA esters. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DECR1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109608

GTX109608 ICC/IF Image

DECR1 antibody [N1C3] detects DECR1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DECR1 protein stained by DECR1 antibody [N1C3] (GTX109608) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DECR1 antibody [N1C3] used in Western Blot (WB). GTX109608

GTX109608 WB Image

Sample (20 μg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX109608 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DECR1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109608

GTX109608 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using DECR1(GTX109608) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DECR1 antibody [N1C3] used in Western Blot (WB). GTX109608

GTX109608 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX109608 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DECR1 antibody [N1C3] used in Western Blot (WB). GTX109608

GTX109608 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DECR1 antibody (GTX109608) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Borgo C et al. Sci Rep 2017; 7 : 42409 Generation and quantitative proteomics analysis of CK2α/α'(-/-) cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for DECR1 antibody [N1C3] (GTX109608). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169