*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , HepG2 , HCT116
Rat, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human DECR2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
2,4-Dienoyl-Coa Reductase 2,Pdcr,Sdr17C1,Decr2
Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.
GTX115788 ICC/IF Image
DECR2 antibody detects DECR2 protein at peroxisome by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DECR2 protein stained by DECR2 antibody (GTX115788) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX115788 WB Image
Sample (30 ug of whole cell lysate)
12% SDS PAGE
GTX115788 diluted at 1:1000
GTX115788 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DECR2(GTX115788) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min