Untreated (–) and treated (+) mouse leukocyte extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DEDD antibody (GTX111882) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DEDD(GTX111882) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.