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DENND1B antibody [N1C1]

Anti-DENND1B antibody [N1C1] used in Western Blot (WB). GTX107160
Anti-DENND1B antibody [N1C1] used in Western Blot (WB). GTX107160
Anti-DENND1B antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107160

Cat. No. GTX107160

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DENND1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Mouse brain , Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DENND1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950111

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DENN domain containing 1B , C1ORF18 , C1orf218 , FAM31B

Background

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM]

Database

DATA IMAGES

Anti-DENND1B antibody [N1C1] used in Western Blot (WB). GTX107160

GTX107160 WB Image

Sample (30 ug of whole cell lysate)
A: MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107160 diluted at 1:1000

Anti-DENND1B antibody [N1C1] used in Western Blot (WB). GTX107160

GTX107160 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107160 diluted at 1:1000

Anti-DENND1B antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107160

GTX107160 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DENND1B(GTX107160) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169